Morphology-driven downscaling of Streptomyces lividans to micro-cultivation

Morphology-driven downscaling of Streptomyces lividans to micro-cultivation

Single Streptomyces lividans K+ Channels

Basic electrophysiological properties of the KcsA K(+) channel were examined in planar lipid bilayer membranes. The channel displays open-state rectification and weakly voltage-dependent gating. Tetraethylammonium blocking affinity depends on the side of the bilayer to which the blocker is added. Addition of Na(+) to the trans chamber causes block of open-channel current, while addition to the ...

Histone-like protein of Streptomyces lividans

A DNA-bineting protein (about lOkDa and pI>9.7) of Streptomyces lividans TK24 was purified on a denatured DNA-Cellulose column, and then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein had high homology with those of small basic DNA-binding proteins known as histone-like proteins. Thus, this protein was designated HSI (histone-like protein of ::J.. lividans)...

Characterization of the Streptomyces lividans PspA response.

Phage shock protein (Psp) is induced by extracytoplasmic stress that may reduce the energy status of the cell. It is encoded in Escherichia coli by the phage shock protein regulon consisting of pspABCDE and by pspF and pspG. The phage shock protein system is highly conserved among a large number of gram-negative bacteria. However, many bacterial genomes contain only a pspA homologue but no homo...

Twin-arginine translocation pathway in Streptomyces lividans.

The recently discovered bacterial twin-arginine translocation (Tat) pathway was investigated in Streptomyces lividans, a gram-positive organism with a high secretion capacity. The presence of one tatC and two hcf106 homologs in the S. lividans genome together with the several precursor proteins with a twin-arginine motif in their signal peptide suggested the presence of the twin-arginine transl...